Students arrived at the lab where their previously learned general knowledge of DNA and protein synthesis was tested by Mr. Petracca, the lab instructor.  The students' recall was impressive.  Mr. Ronald Schollenberger their science teacher, had informed them in class and via their Assignment that they were about to embark on a more in-depth study about gene splicing and genetic engineering technology, so this was perfect timing for the impending study and the students were well prepared.

Mr. Petracca demonstrated each step of the procedure required to split and isolate the pieces of DNA. Once the pieces were separated, the students were asked to identify the "unknown restriction enzyme" that was used by comparing the separation patterns of the known enzymes with the unknown.

Splicing DNA has allowed scientists to engineer many breakthroughs which have been used to improve food sources, to serve as a source of many medicines, and to help to control/cure some diseases. The science of genetic engineering has the potential for a further and profound impact on medicine in the future.

Working with all of the necessary lab apparatus and chemicals to stain the DNA samples, the students micropipetted the stained DNA pieces into their agar gel plates and then added an electric current which caused the DNA strands to travel various distances through the gel. While they waited for the process to advance, the group took a break to view the museum exhibition which displays the history and other interesting aspects of DNA research.

Once the gels were ready, digital photos were taken of each gel and discussed with the class. After their departure, Mr. Petracca placed the gels back in their electrically charged baths to allow the fragments to separate even further, allowing for a clearer view of the results of the electrophoresis. He posted them on the lab's site for the students to view at home.