During this experiment, a piece of DNA is spliced into multiple pieces using various restriction enzymes, and then the pieces are separated from each other utilizing a process known as electrophoresis.

On the morning of Thursday, October 28, the two sections of students arrived at the lab where their previously learned general knowledge of DNA and protein synthesis was tested by Dr. Nash, the lab instructor.  The students' recall was impressive. Their enthusiastic Dalton science teacher, Mr. Ronald Schollenberger, had informed them in class and via their Assignment that their Advanced Bio classes were  about to embark on a more in-depth study about gene splicing and genetic engineering technology, so this was perfect timing for the impending study and the students were well prepared.

Dr. Nash, their lab instructor, demonstrated each step of the procedure required to split and isolate the pieces of DNA. Once the pieces were separated, the students were asked to identify the "unknown restriction enzyme" that was used by comparing the separation patterns of the known enzymes with the unknown.

Splicing DNA has allowed scientists to engineer many breakthroughs which have been used to improve food sources, to serve as a source of many medicines, and to help to control/cure a number of diseases. The science of genetic engineering has great potential for a further and  profound impact on medicine in the future.

Working with all of the necessary lab apparatus and chemicals to stain the DNA samples, the students micropipetted the stained DNA pieces into their agar gel plates and then added an electric current which caused the DNA strands to travel various distances through the gel. While they waited for the process to advance, the group took a break to view the museum exhibition which displays the history and other interesting aspects of DNA research. Once the gels were ready, Dr. Nash took digital photos of each gel and discussed them with the class. After their departure, Dr. Nash placed the gels back in their electrically charged baths to allow the fragments to separate out even further, allowing for a clearer view of the results of the electrophoresis.  Later he posted them on the lab's site for the students to view at home.

Before heading back, the hungry students and their teacher shared an excellent deli lunch, and the fall sun and colors in a local park along  Cold Spring Harbor's harbor.